ANTIBIOGRAM OF PRE-PROCESSED RAW CHICKEN MEAT FROM DIFFERENT SUPERSHOPS OF DHAKA CITY, BANGLADESH
Alam, Seemi Tasnim, el at.
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Pathogenic strains of salmonella, S. aureus, S. epidermidis, shigella, enterobacter and citrobacter are serious health threat for human being. Present investigation was conducted to assess the antibiogram of pre-processed raw chicken meat collected from different chain-shops of Dhaka city. Pre-processed chicken meat samples were collected from three different super-shops (S1, S2, and S3) in Dhaka. The Samples are serially diluted to 10-4, and after growth on MSA, MAC, and SS agars, the total counts showed The most growth S2 (360 × 10.4 18.33), (235 × 10.4), (25 × 10.14.4 3.6) followed by S3 (353.3 × 10.4 42.39), (151.67 × 10.4 22.19), 15 × 10-4 3.0) and finally S1 (3.26.67 × 10.4 29.48), (64.5 × 10.4 8.62) (14 × 10.4 5.13) respectively. Salmonella S aureus, S. epidermidis, Shigella, Enterobacter and Citrobacter were identified after a number of morphological and biochemical tests; Gram staining, MIU, KIA, oxidase and catalase tests, and citrate utilization test following aseptic techniques. Six antibiotics; Vancomycin, and five broad spectrum drugs; Streptomycin, Norfloxacin, Novobiocin, Chloramphenicol, and Cefotaxime were used to test the sensitivity or resistance of the organisms. All the pathogens were susceptible to chloramphenicol. Staphylococcusepidermidis were novobiocin-resistant. Citrobacter spp. and Enterobacter spp. were sensitive to all except novobiocin. Staphylococcusepidermidis was susceptible and intermediate to norfloxacin. Staphylococcusaureus was resistant to norfloxacin and novobiocin; intermediate to cefotaxime, and sensitive to the rest. Salmonella spp. was found to be resistant to novobiocin, vancomycin, and streptomycin; intermediate to norfloxacin, but sensitive to chloramphenicol and cefotaxime. Shigella.spp was found to be sensitive to all the antibiotics but resistant to novobiocin and vancomycin. Fecal coliform E. coli was absent showing some degree of sanitation in the chicken, and all the pathogens were found susceptible to at least two antibiotics.